Journal: iScience

Article Title: Identification of IQCH as a calmodulin-associated protein required for sperm motility in humans

doi: 10.1016/j.isci.2023.107354

Figure Lengend Snippet: Analysis of the spatial distribution of IQCH within sperm flagella by super-resolution microscopy (A) STORM imaging of sperm flagella stained for IQCH (red) and α-tubulin (green) or calmodulin (CaM, green). Scale bars 1 μm. (B) STED images of co-staining IQCH (violet) and α-tubulin (green), highlighting the helicoidal spatial distribution of IQCH along the sperm flagellum (dashed frames) and its co-localization with calmodulin. Distribution profiles of the signal in the selected regions highlight the longitudinal and transversal periodicity of IQCH. Scale bars 2 μm and 2.5 μm. (C) Schematic representation of a transversal section of a sperm flagellum with the localization of proteins identifying the various complexes and subcellular regions of sperm flagella localization [Graphical elements from Servier Medical Art, licensed under a Creative Commons Attribution 3.0 Unported License]. Distribution data were obtained by analyzing the STORM and STED images shown in Figure S4 .

Article Snippet: Mounting buffer kit for STORM imaging , Abbelight , Smart kit.

Techniques: Super-Resolution Microscopy, Imaging, Staining

Journal: iScience

Article Title: Identification of IQCH as a calmodulin-associated protein required for sperm motility in humans

doi: 10.1016/j.isci.2023.107354

Figure Lengend Snippet:

Article Snippet: Mounting buffer kit for STORM imaging , Abbelight , Smart kit.

Techniques: Control, Recombinant, Protease Inhibitor, Electron Microscopy, Plasmid Preparation, Reverse Transcription, Gel Extraction, SYBR Green Assay, Imaging, In Situ, Software